Under technogenic pollution, phytotoxicity of heavy metals (HM) becomes a factor limiting yield and quality of crop production. In breeding, an intraspecific polymorphism of resistance to technogenic factors should be estimated with the analysis of its formation and maintenance. Using spring barley (Hordeum vulgare L.) Zazerskii 85, Gorinskii and Chelyabinskii 1 varieties, we studied the influence of different Pb(NO3)2 concentrations (1.0; 1.5; 2.0; 2.5; 3.0; 3.5; 4.0; 4.5; 5.0 mg/ml) on the growth of roots and offsprings in seedlings. Then, a testing concentration of Pb(NO3)2 found out was applied to investigate an intraspecific polymorphism of barley plant tolerance to the toxicant.