Potato virus X (Potexvirus) is an important pathogen of number of economically significant agricultural plants of the Solanaceae family. Potato virus X (PVX) virions are flexible filamentous particles 515 nm in length and 13.5 nm in diameter with a helical structure. PVX genome consists of a single-stranded RNA (6435 nucleotides) which is capped and polyadenylated. The study of various stages of the infection process of plant viruses during infection, including the assembly of viral particles is of great practical interest. Identification of these mechanisms can be the basis for developing new approaches in virus-free crop production. Herein, the initial stages of potato virus X (PVX) virion assembly were examined on the example of virus-like particles (VLPs) produced by incubation of PVX RNA with PVX coat protein (CP) in vitro.