РУсскоязычный Архив Электронных СТатей периодических изданий
Agricultural Biology/2012/№ 6/

METHODS FOR SANITARY SURVEILLANCE OF LIVESTOCK PRODUCTION. VII. ENZYMOIMMUNOASSAY OF STREPTOMYCIN

The polyclonal rabbit antibodies against streptomycin conjugated with bovine serum albumin in reaction with diglycidyl ether of 1,4-butanediol presents high specificity to streptomycin and its similar structural analog — dihydrostreptomycin. In the conditions of indirect competitive enzymoimmunoassay with immobilized antigens, heterologous to immunogene on protein carrier and methods of synthesis, the limit of streptomycin detection is 0.1 ng/ml. The authors consider the use of developed immunoenzyme test-systems for control of antibiotic contamination in milk and eggs with the limit of detection of 10 mkg/kg and in meat with the limit of 20 mkg/kg.

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