DIAGNOSTIC VALUE OF A TEST FOR DETECTION OF SECRETORY IgA FOR CONFIRMATION OF ABSENCE OF FMD VIRUS CIRCULATION

In Russia the preventive vaccination dominates over other protective and preventive measures against foot and mouth disease (FMD). However, the development of the subclinical infection in immune animals should not be ruled out as these animals become virus carriers and pose a potential threat for susceptible animal population. Therefore, the design and reduction to practice of tests for identification of virus-carrier animals continues to be relevant. Secretory immunoglobulins A (sIgA) provide the first line of protection against many infectious agents, they are capable of inhibiting virus intracellular replication and serve as a transmitter of virus neutralization. As reported by foreign authors there is a possibility to identify virus-carrier animals using a rapid ELISA-based test detecting secretory IgA (sIgA-ELISA). Previously we determined sIgA-ELISA optimal conditions for detection virus-specific sIgA in saliva samples in one dilution. In the given paper results of validation and evaluation of the sIgA-ELISA test-system («sandwich» ELISA) are shown as compared with other laboratory methods. The paper presents data on testing bovine biological samples (96 animals with body weight of 200-300 kg at the age of 18-24 months) from FMD-free agricultural enterprises (Vladimir Oblast) collected before and after vaccination from animals, immunized with different batches of FMD vaccine and subject to experimental infection.

• Afonina D.N.
• Kamalova N.Ye.
• Kan’Hina A.V.
• Timina A.M.

• Искусство. Декоративно-прикладное искусство. Фотография. Музыка. Игры. Спорт

• Естественные науки
• Сельское хозяйство. Лесоводство