Evaluation of some microsatellite markers variability in the study of genetic structure of vendace (Coregonus albula (L.)) populations from Latvian Lakes

Coregonus albula microsatellites Latvian lakes polymorphism heterozygosity differentiation.

Генетическая дифференциация сиговых рыб в реке Печоре, Динамика популяции европейской ряпушки Coregonus albula Сямозера (Республика Карелия), ...

Сибирский экологический журнал, 2 (2016) 184193 УДК 597.2/.5 DOI 10.15372/SEJ20160202 Evaluation of some microsatellite markers variability in the study of genetic structure of vendace (Coregonus albula (L.)) populations from Latvian Lakes N. KUTE, J. OREHA Institute of Life Sciences and Technology Daugavpils University LV-5400, Daugavpils, Parades str., 1A E-mail: nàtalja.skute@du.lv, jelenà.oreha@du.lv Статья поступила 21.05.2015 Принята к печати 16.10.2015 ABSTRACT Vendace (Coregonus albula (L.)) is a very plastic species of freshwater whitefish which is widespread in Europe. <...> We examined cross-species amplification of 14 microsatellite loci (Cocl-Lav22, Cocl-Lav23, Cisco-59, Cisco-106, Cisco-90, Cisco-126, Cisco-157, Cisco-179, Cisco-181, Cisco-183, Cisco-200, BWF1, BWF2, and C4-183) which were successfully used for genetic studies, monitoring, protection and management of different Coregonus species. <...> Five microsatellite markers (BWF1, Cisco-90, Cisco-126, Cisco-157, Cisco-200) had a good representation in populations of vendace from five Latvian lakes (Sventes, Raznas, Nirzas, Dridzas, Stirnu) and were used for genetic analysis of these populations. <...> It was shown that these 5 microsatellite loci were polymorphic and could differ among the investigated populations. <...> The mean number of alleles per locus (from 4.4 to 6.2), the observed (from 0.53 to 0.70) and expected (from 0.49 to 0.70) heterozygosity, population structure (FST and RST) and gene flow (Nm) were also analysed in Latvian vendace populations. <...> The vendace (Coregonus albula) is a fish widely spread in Holarctic waters. <...> Therefore the aim of this work was to evaluate the variability of some microsatellite markers and use them to study the genetic structure of vendace populations in Latvian lakes. # MATERIALS AND METHODS The fish samples were collected in Latvia in 2007, with the help of specialists of the Latvian Fish Resources Agency, from sampling catches according to Latvian Fish Resources Agency monitoring plan from seven Latvian lakes: Sventes, Raznаs, Nirzas, Dridzas, Alu nes and Eezers. <...> The six microsatellite primers (Cisco-90, Cisco-106, Cisco-126, Cisco-157, Cisco-200 and BWF1), which gave repeatable and informative responds in testing, were selected for analysis. <...> Number of alleles in a locus, frequency, private alleles in each population, observed and expected heterozygosity level in polymorph <...>

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