Introduction. The genes encoding the HLA-DQ heterodimer molecules, DQA1 and DQB1, have been found to have the association with Lyme borreliosis (LB) risk, although there is cumulative evidence for the effect of other gene loci within the major histocompatibility complex gene region. Objective. The purpose of this study was to determine of HLA DQA1/DQB1 haplotypes in patients with clinical, epidemiological and laboratory approved Lyme borreliosis diagnosis